Shotgun Metagenomic Profiling
Species-level composition, diversity, rarefaction and microbial-community structure.
Explore example →Microbiome & metagenomics
Microbiome & metagenomics · 16S/ITS and shotgun
Microbial-community profiling with a clear separation between 16S/ITS amplicon sequencing and shotgun metagenomics.
Amplicon microbiome profiling · 16S/ITS
Marker-gene approach for community composition and diversity.
- Filtering and denoising
- ASV generation
- Marker-appropriate taxonomic assignment
- Alpha/beta diversity, rarefaction and ordination
This approach mainly characterises community composition and diversity; it does not directly measure global functional potential.
Shotgun metagenomics
Untargeted sequencing for broader taxonomic profiling and direct functional characterisation when supported by the data.
- QC and host removal when required
- Taxonomic profiling
- Pathways and functional potential
- Resistome, assembly and MAGs depending on depth and project
Statistical comparison
Diversity, PERMANOVA, differential abundance and compositionality-aware models compare communities without reducing inference to raw percentages.
Results provided
ASV tables or taxonomic profiles, diversity metrics, statistical results, functional annotations where relevant, figures, methods and interactive reporting.
Reporting examples
Functional Metagenomic Profiling
Pathways, functional PCoA and differential comparison.
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